Coding

Part:BBa_K4257011:Design

Designed by: Ye Gu   Group: iGEM22_CPU_Nanjing   (2022-09-26)


RPD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RPD will be overexpressed using medium-copy number plasmid pBBR1MCS2.


Source

The RPD coding sequence was commercially synthesized after codon optimization.

References

Hirota, R., Yamane, S.-t., Fujibuchi, T., Motomura, K., Ishida, T., Ikeda, T. and Kuroda, A. (2012) Isolation and characterization of a soluble and thermostable phosphite dehydrogenase from Ralstonia sp. strain 4506. Journal of Bioscience Bioengineering 113(4), 445-450.